human kyse180 escc cell line  (Procell Inc)

Journal: Cell Reports Medicine

Article Title: Macrophage efferocytosis mediated by the TP63-RAC2 pathway promotes immunosuppressive remodeling in esophageal cancer

doi: 10.1016/j.xcrm.2025.102529

Figure Lengend Snippet: Identification of ESCC cell types based on specific markers among 9 clusters (A) Two-dimensional (2D) plots of UMAP dimensionality reduction of samples from different sources in single-cell transcriptome data for 121,038 high-quality cells. (B) Cluster analysis was performed using the Seurat package to label the different cell types. (C and D) UMAP plots showed 9 ESCC cell types upon re-clustering. The pie charts showed the distribution of 9 cell types in different phases (C) and different groups (D). (E and F) The bar graphs showed the proportion of each cell type in the tumor (T) and lymph node (LN) groups. (G) Expression of top 5 marker genes for each cell type and their expression in different groups (T and LN) and different phases (G1, S, and G2M). (H) Violin plots showed the different values of different cell types in nFeature-RNA, nCount-RNA, G2M value, and S value. (I) 2D plots of UMAP dimensionality reduction of nFeature-RNA, nCount-RNA, G2M value, and S value.

Article Snippet: Human: KYSE180 ESCC cell line , Procell , CL-0760.

Techniques: Expressing, Marker

Journal: Cell Reports Medicine

Article Title: Macrophage efferocytosis mediated by the TP63-RAC2 pathway promotes immunosuppressive remodeling in esophageal cancer

doi: 10.1016/j.xcrm.2025.102529

Figure Lengend Snippet: The role of macrophages in the activity of efferocytosis (A) The UMAP plots showed macrophages from the T and LN groups, respectively. (B) AUC values for efferocytosis activity in different macrophage subtypes. Colors that tend to be yellow indicate higher AUC values, and colors that tend to be bluer indicate lower AUC values. (C) Comparison of AUC values for cell efferocytosis in different groups and phases. The Wilcoxon test was used for comparison between two groups, and the Kruskal test was used for comparison between multiple groups. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, and ns indicates no statistical difference. (D) Comparison of AUC values of macrophage efferocytosis activity in ESCC tumor samples and normal samples from the GEO database. An unpaired two-sided Wilcoxon test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, and ns indicates no statistical difference. (E) Differential expression of macrophage efferocytosis-related genes in ESCC tumor samples and normal samples from the GEO database. (F and G) AUC values of efferocytosis activity and efferocytosis-related gene expression in the different macrophage subtypes. An unpaired two-sided Wilcoxon test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, and ns indicates no statistical difference. (H) Score of M1- and M2-related genes in different macrophage subtypes and groups. An unpaired two-sided t test was used. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, and ns indicates no statistical difference. (I) Expression of efferocytosis genes in each macrophage subtype and different groups, different phases, G2M values, S values, and nCount-RNA. (J and K) Expression levels and distribution of three representative efferocytosis genes, TGM2, AXL, and ABCG1, in different macrophage subtypes.

Article Snippet: Human: KYSE180 ESCC cell line , Procell , CL-0760.

Techniques: Activity Assay, Comparison, Quantitative Proteomics, Gene Expression, Expressing